Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus
Identifieur interne : 005310 ( Main/Exploration ); précédent : 005309; suivant : 005311Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus
Auteurs : Chia-Jung Yu ; Yee-Chun Chen ; Cheng-Hsiang Hsiao ; Tse-Chun Kuo ; Shin C. Chang ; Chun-Yi Lu ; Wen-Chin Wei ; Chia-Huei Lee ; Li-Min Huang ; Ming-Fu Chang ; Hong-Nerng Ho ; Fang-Jen S. LeeSource :
- Febs Letters [ 0014-5793 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN complémentaire (métabolisme), Alvéoles pulmonaires (virologie), Animaux, Anticorps (), Appareil de Golgi (métabolisme), Cadres ouverts de lecture, Cellules Vero, Clonage moléculaire, Cytochromes de type b (), Cytoplasme (métabolisme), Escherichia coli (métabolisme), Facteurs temps, Fractions subcellulaires (métabolisme), Humains, Immunohistochimie, Lapins, Microscopie de fluorescence, Nucléocapside (métabolisme), Peptides (), Poumon (virologie), Protéines recombinantes (), Protéines recombinantes (métabolisme), Protéines virales (), Structure tertiaire des protéines, Virus du SRAS (métabolisme), Électrophorèse sur gel de polyacrylamide.
- MESH :
- métabolisme : ADN complémentaire, Appareil de Golgi, Cytoplasme, Escherichia coli, Fractions subcellulaires, Nucléocapside, Protéines recombinantes, Virus du SRAS.
- virologie : Alvéoles pulmonaires, Poumon.
- Animaux, Anticorps, Cadres ouverts de lecture, Cellules Vero, Clonage moléculaire, Cytochromes de type b, Facteurs temps, Humains, Immunohistochimie, Lapins, Microscopie de fluorescence, Peptides, Protéines recombinantes, Protéines virales, Structure tertiaire des protéines, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Animals, Antibodies (chemistry), Chlorocebus aethiops, Cloning, Molecular, Cytochrome b Group (chemistry), Cytoplasm (metabolism), DNA, Complementary (metabolism), Electrophoresis, Polyacrylamide Gel, Escherichia coli (metabolism), Golgi Apparatus (metabolism), Humans, Immunohistochemistry, Lung (virology), Microscopy, Fluorescence, Nucleocapsid (metabolism), Open Reading Frames, Peptides (chemistry), Protein Structure, Tertiary, Pulmonary Alveoli (virology), Rabbits, Recombinant Proteins (chemistry), Recombinant Proteins (metabolism), SARS Virus (metabolism), Subcellular Fractions (metabolism), Time Factors, Vero Cells, Viral Proteins (chemistry).
- MESH :
- chemical , chemistry : Antibodies, Cytochrome b Group, Peptides, Recombinant Proteins, Viral Proteins.
- metabolism : Cytoplasm, DNA, Complementary, Escherichia coli, Golgi Apparatus, Nucleocapsid, Recombinant Proteins, SARS Virus, Subcellular Fractions.
- virology : Lung, Pulmonary Alveoli.
- Animals, Chlorocebus aethiops, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Humans, Immunohistochemistry, Microscopy, Fluorescence, Open Reading Frames, Protein Structure, Tertiary, Rabbits, Time Factors, Vero Cells.
Abstract
The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS‐CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261–274 of the putative protein. Anti‐P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in
Url:
DOI: 10.1016/j.febslet.2004.03.086
PubMed: 15135062
PubMed Central: 7126674
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000874
- to stream Pmc, to step Curation: 000874
- to stream Pmc, to step Checkpoint: 001640
- to stream PubMed, to step Corpus: 002E07
- to stream PubMed, to step Curation: 002E07
- to stream PubMed, to step Checkpoint: 002C65
- to stream Ncbi, to step Merge: 000801
- to stream Ncbi, to step Curation: 000801
- to stream Ncbi, to step Checkpoint: 000801
- to stream Main, to step Merge: 005722
- to stream Main, to step Curation: 005310
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus</title><author><name sortKey="Yu, Chia Jung" sort="Yu, Chia Jung" uniqKey="Yu C" first="Chia-Jung" last="Yu">Chia-Jung Yu</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Yee Chun" sort="Chen, Yee Chun" uniqKey="Chen Y" first="Yee-Chun" last="Chen">Yee-Chun Chen</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF2"></nlm:aff></affiliation></author><author><name sortKey="Hsiao, Cheng Hsiang" sort="Hsiao, Cheng Hsiang" uniqKey="Hsiao C" first="Cheng-Hsiang" last="Hsiao">Cheng-Hsiang Hsiao</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF3"></nlm:aff></affiliation></author><author><name sortKey="Kuo, Tse Chun" sort="Kuo, Tse Chun" uniqKey="Kuo T" first="Tse-Chun" last="Kuo">Tse-Chun Kuo</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF2"></nlm:aff></affiliation><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF4"></nlm:aff></affiliation></author><author><name sortKey="Chang, Shin C" sort="Chang, Shin C" uniqKey="Chang S" first="Shin C." last="Chang">Shin C. Chang</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF5"></nlm:aff></affiliation></author><author><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF6"></nlm:aff></affiliation></author><author><name sortKey="Wei, Wen Chin" sort="Wei, Wen Chin" uniqKey="Wei W" first="Wen-Chin" last="Wei">Wen-Chin Wei</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF7"></nlm:aff></affiliation></author><author><name sortKey="Lee, Chia Huei" sort="Lee, Chia Huei" uniqKey="Lee C" first="Chia-Huei" last="Lee">Chia-Huei Lee</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF7"></nlm:aff></affiliation></author><author><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF6"></nlm:aff></affiliation></author><author><name sortKey="Chang, Ming Fu" sort="Chang, Ming Fu" uniqKey="Chang M" first="Ming-Fu" last="Chang">Ming-Fu Chang</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF7"></nlm:aff></affiliation></author><author><name sortKey="Ho, Hong Nerng" sort="Ho, Hong Nerng" uniqKey="Ho H" first="Hong-Nerng" last="Ho">Hong-Nerng Ho</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF1"></nlm:aff></affiliation><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF8"></nlm:aff></affiliation></author><author><name sortKey="Lee, Fang Jen S" sort="Lee, Fang Jen S" uniqKey="Lee F" first="Fang-Jen S." last="Lee">Fang-Jen S. Lee</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF1"></nlm:aff></affiliation><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF4"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15135062</idno><idno type="pmc">7126674</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126674</idno><idno type="RBID">PMC:7126674</idno><idno type="doi">10.1016/j.febslet.2004.03.086</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000874</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000874</idno><idno type="wicri:Area/Pmc/Curation">000874</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000874</idno><idno type="wicri:Area/Pmc/Checkpoint">001640</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001640</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15135062</idno><idno type="wicri:Area/PubMed/Corpus">002E07</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002E07</idno><idno type="wicri:Area/PubMed/Curation">002E07</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002E07</idno><idno type="wicri:Area/PubMed/Checkpoint">002C65</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002C65</idno><idno type="wicri:Area/Ncbi/Merge">000801</idno><idno type="wicri:Area/Ncbi/Curation">000801</idno><idno type="wicri:Area/Ncbi/Checkpoint">000801</idno><idno type="wicri:doubleKey">0014-5793:2004:Yu C:identification:of:a</idno><idno type="wicri:Area/Main/Merge">005722</idno><idno type="wicri:Area/Main/Curation">005310</idno><idno type="wicri:Area/Main/Exploration">005310</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus</title><author><name sortKey="Yu, Chia Jung" sort="Yu, Chia Jung" uniqKey="Yu C" first="Chia-Jung" last="Yu">Chia-Jung Yu</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Yee Chun" sort="Chen, Yee Chun" uniqKey="Chen Y" first="Yee-Chun" last="Chen">Yee-Chun Chen</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF2"></nlm:aff></affiliation></author><author><name sortKey="Hsiao, Cheng Hsiang" sort="Hsiao, Cheng Hsiang" uniqKey="Hsiao C" first="Cheng-Hsiang" last="Hsiao">Cheng-Hsiang Hsiao</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF3"></nlm:aff></affiliation></author><author><name sortKey="Kuo, Tse Chun" sort="Kuo, Tse Chun" uniqKey="Kuo T" first="Tse-Chun" last="Kuo">Tse-Chun Kuo</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF2"></nlm:aff></affiliation><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF4"></nlm:aff></affiliation></author><author><name sortKey="Chang, Shin C" sort="Chang, Shin C" uniqKey="Chang S" first="Shin C." last="Chang">Shin C. Chang</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF5"></nlm:aff></affiliation></author><author><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF6"></nlm:aff></affiliation></author><author><name sortKey="Wei, Wen Chin" sort="Wei, Wen Chin" uniqKey="Wei W" first="Wen-Chin" last="Wei">Wen-Chin Wei</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF7"></nlm:aff></affiliation></author><author><name sortKey="Lee, Chia Huei" sort="Lee, Chia Huei" uniqKey="Lee C" first="Chia-Huei" last="Lee">Chia-Huei Lee</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF7"></nlm:aff></affiliation></author><author><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF6"></nlm:aff></affiliation></author><author><name sortKey="Chang, Ming Fu" sort="Chang, Ming Fu" uniqKey="Chang M" first="Ming-Fu" last="Chang">Ming-Fu Chang</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF7"></nlm:aff></affiliation></author><author><name sortKey="Ho, Hong Nerng" sort="Ho, Hong Nerng" uniqKey="Ho H" first="Hong-Nerng" last="Ho">Hong-Nerng Ho</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF1"></nlm:aff></affiliation><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF8"></nlm:aff></affiliation></author><author><name sortKey="Lee, Fang Jen S" sort="Lee, Fang Jen S" uniqKey="Lee F" first="Fang-Jen S." last="Lee">Fang-Jen S. Lee</name><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF1"></nlm:aff></affiliation><affiliation><nlm:aff id="feb2s0014579304003928-aff-AFF4"></nlm:aff></affiliation></author></analytic><series><title level="j">Febs Letters</title><idno type="ISSN">0014-5793</idno><idno type="eISSN">1873-3468</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies (chemistry)</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Cytochrome b Group (chemistry)</term><term>Cytoplasm (metabolism)</term><term>DNA, Complementary (metabolism)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Escherichia coli (metabolism)</term><term>Golgi Apparatus (metabolism)</term><term>Humans</term><term>Immunohistochemistry</term><term>Lung (virology)</term><term>Microscopy, Fluorescence</term><term>Nucleocapsid (metabolism)</term><term>Open Reading Frames</term><term>Peptides (chemistry)</term><term>Protein Structure, Tertiary</term><term>Pulmonary Alveoli (virology)</term><term>Rabbits</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (metabolism)</term><term>Subcellular Fractions (metabolism)</term><term>Time Factors</term><term>Vero Cells</term><term>Viral Proteins (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (métabolisme)</term><term>Alvéoles pulmonaires (virologie)</term><term>Animaux</term><term>Anticorps ()</term><term>Appareil de Golgi (métabolisme)</term><term>Cadres ouverts de lecture</term><term>Cellules Vero</term><term>Clonage moléculaire</term><term>Cytochromes de type b ()</term><term>Cytoplasme (métabolisme)</term><term>Escherichia coli (métabolisme)</term><term>Facteurs temps</term><term>Fractions subcellulaires (métabolisme)</term><term>Humains</term><term>Immunohistochimie</term><term>Lapins</term><term>Microscopie de fluorescence</term><term>Nucléocapside (métabolisme)</term><term>Peptides ()</term><term>Poumon (virologie)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines virales ()</term><term>Structure tertiaire des protéines</term><term>Virus du SRAS (métabolisme)</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies</term><term>Cytochrome b Group</term><term>Peptides</term><term>Recombinant Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cytoplasm</term><term>DNA, Complementary</term><term>Escherichia coli</term><term>Golgi Apparatus</term><term>Nucleocapsid</term><term>Recombinant Proteins</term><term>SARS Virus</term><term>Subcellular Fractions</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN complémentaire</term><term>Appareil de Golgi</term><term>Cytoplasme</term><term>Escherichia coli</term><term>Fractions subcellulaires</term><term>Nucléocapside</term><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Alvéoles pulmonaires</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term><term>Pulmonary Alveoli</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Humans</term><term>Immunohistochemistry</term><term>Microscopy, Fluorescence</term><term>Open Reading Frames</term><term>Protein Structure, Tertiary</term><term>Rabbits</term><term>Time Factors</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps</term><term>Cadres ouverts de lecture</term><term>Cellules Vero</term><term>Clonage moléculaire</term><term>Cytochromes de type b</term><term>Facteurs temps</term><term>Humains</term><term>Immunohistochimie</term><term>Lapins</term><term>Microscopie de fluorescence</term><term>Peptides</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Structure tertiaire des protéines</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS‐CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261–274 of the putative protein. Anti‐P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in <italic>Escherichia coli</italic> and in Vero E6 cells. Expression of SARS protein 3a was detected at 8–12 h after infection and reached a higher level after ∼24 h in SARS‐CoV‐infected Vero E6 cells. Protein 3a was also detected in the alveolar lining pneumocytes and some intra‐alveolar cells of a SARS‐CoV‐infected patient's lung specimen. Recombinant protein 3a expressed in Vero E6 cells and protein 3a in the SARS‐CoV‐infected cells was distributed over the cytoplasm in a fine punctate pattern with partly concentrated staining in the Golgi apparatus. Our study demonstrates that SARS‐CoV indeed expresses a novel protein 3a, which is present only in SARS‐CoV and not in other known CoVs.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Knipe, D M" uniqKey="Knipe D">D.M. Knipe</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chang, Ming Fu" sort="Chang, Ming Fu" uniqKey="Chang M" first="Ming-Fu" last="Chang">Ming-Fu Chang</name><name sortKey="Chang, Shin C" sort="Chang, Shin C" uniqKey="Chang S" first="Shin C." last="Chang">Shin C. Chang</name><name sortKey="Chen, Yee Chun" sort="Chen, Yee Chun" uniqKey="Chen Y" first="Yee-Chun" last="Chen">Yee-Chun Chen</name><name sortKey="Ho, Hong Nerng" sort="Ho, Hong Nerng" uniqKey="Ho H" first="Hong-Nerng" last="Ho">Hong-Nerng Ho</name><name sortKey="Hsiao, Cheng Hsiang" sort="Hsiao, Cheng Hsiang" uniqKey="Hsiao C" first="Cheng-Hsiang" last="Hsiao">Cheng-Hsiang Hsiao</name><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name><name sortKey="Kuo, Tse Chun" sort="Kuo, Tse Chun" uniqKey="Kuo T" first="Tse-Chun" last="Kuo">Tse-Chun Kuo</name><name sortKey="Lee, Chia Huei" sort="Lee, Chia Huei" uniqKey="Lee C" first="Chia-Huei" last="Lee">Chia-Huei Lee</name><name sortKey="Lee, Fang Jen S" sort="Lee, Fang Jen S" uniqKey="Lee F" first="Fang-Jen S." last="Lee">Fang-Jen S. Lee</name><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name><name sortKey="Wei, Wen Chin" sort="Wei, Wen Chin" uniqKey="Wei W" first="Wen-Chin" last="Wei">Wen-Chin Wei</name><name sortKey="Yu, Chia Jung" sort="Yu, Chia Jung" uniqKey="Yu C" first="Chia-Jung" last="Yu">Chia-Jung Yu</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005310 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005310 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:7126674
|texte= Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15135062" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |